Composite

Part:BBa_K3036004:Design

Designed by: Xiao Chen,Chunxu Han   Group: iGEM19_BNU-China   (2019-10-09)


Cold triggered kill switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cold-triggered kill switch is characterized in a system where the downstream relE (BBa_K185000) gene encodes for a constantly expressed stable toxin and the upstream relB (BBa_K185048) gene encodes for a labile antitoxin under the control of the RNA thermometer (BBa_K115002).


Source

This part derives from Escherichia.coli DH5alpha.

References